The research project uses an innovative approach to gain as much as achievable information within our resources. This has led to an experimental approach using aircraft engine simulations, and mostly animal-free toxicity tests to gain a better understanding about cabin air quality and possible health risks. Without a full understanding of the substances present in the cabin air and possible related (mechanisms of) diseases, deliberately exposing human volunteers would be highly inappropriate. Due to the complexity of the composition of cabin air, an experimental approach is favored over an observational epidemiological study.